Early life aversive experiences influence brain development, contributing to the emergence of psychiatric diseases, such as schizophrenia and major depression. Most of the inhibitory neurons in the thalamus are parvalbumin-expressing (PV+) cells located in the thalamic reticular nucleus (TRN). Alterations of PV+ interneurons, some of them mediated by perineuronal nets (PNNs), have been related to these psychiatric diseases. We have developed two models of early life aversive experiences on the TRN of adult mice. We have used a double-hit (DH) model in male mice combining an injection of the NMDAR antagonist MK801 at postnatal day (PD) 7 and post-weaning social isolation. Conversely, we applied a peripubertal stress (PPS) model in male and female mice between PD 28 and 42. Then, we analyzed the expression of PV, PNNs, and NMDARs in the TRN using immunofluorescence staining. We observed, using the DH model, significant differences in the intensity of fluorescence of PV+ cells and PNNs, as well as the density and the area occupied by different NMDA receptor subunits. Regarding the PPS model, we observed an increase in the intensity fluorescence of PNNs and the proportion of PV+ cells surrounded by PNNs exclusively in females. We also found an increase in the density of GluN1 NMDAR on the PV+ cells of females, strongly indicatingting sex-based differences in the effects of PPS on TRN. Our results unveils the involvement of the TRN and PV+ interneurons in early life aversive experiences, as well as highlight sex-based differences in preclinical animal models for psychiatric disorders.