Background: Folate supplementation throughout pregnancy is recommended to support foetal and early postnatal brain development. The exact effect of folate on cerebellar development is unknown. This study looked at how maternal folate consumption impacts the structural development of the offspring's cerebellar cortex.Methods and materials: Adult (6-8 week old) female albino rats were randomly allocated to four groups: folate deficiency (folate 0 mg/kg), regular diet (2 mg/kg), folate enriched (8 mg/kg), and folate supra-enriched (40 mg/kg) premixed meals. The meals were started 14 days before mating and maintained throughout gestation and lactation. On postnatal days 0, 7, 21, and 35, five pups from each group were sacrificed and their cerebellas processed for light microscopy and immunohistochemistry (Ki67, cleaved caspase-3, GFAP, NeuN).Results: Compared to other study groups, folate-deficient diet offspring had smaller cerebellum dimensions, fused folia, dysmorphic cells, lower cell densities, and reduced cortical layer volumes (p<0.05). Folate-enriched groups showed thicker, branching folia, sub-lobule growth, dense cerebellar cortex, and increased volumes in external, molecular, Purkinje, and internal granule cell layers (p<0.05) compared to controls. The folate-supplemented group had far larger cell densities and cerebellar cortical layer volumes than the supra-supplemented group.Conclusion: These findings suggest folate supplementation is necessary for cerebellar cortex development. Folate-enriched groups feature densely packed cells in all cerebellar cortex layers, showing neuronal density and function. The folate-supplemented group had larger cell densities and cerebellar cortex layer diameters than the supra-supplemented group, showing optimal folate consumption for cerebellar development and function.