Advances in the development of microelectrode arrays (MEAs) for in-vitro electrophysiological recordings have enabled the characterization of multi-scale behavior in neuronal networks, ranging from subcellular level to network dynamics. Such devices are fundamental for studying the phenotype of neurological disorders and for drug discovery, providing unique insights into the complexity of neuronal networks. Electrode density, spacing, and size influence the signal quality, noise level, and sensitivity. To properly characterize the full behavior of neuronal networks, MEAs must combine single-cell and subcellular resolution with high-throughput assays, while maintaining sensitivity to small extracellular action potentials to describe the full range of network dynamics. In this study, the MaxOne and MaxTwo high-density (HD) MEA systems (MaxWell Biosystems, Switzerland) were used to record activity from induced pluripotent stem cell derived neurons, demonstrating the advantages of having 26,400 electrodes per well, which is key to increasing the statistical power of data collected longitudinally. HD-MEA recordings were compared with simulated low-density recordings, in which larger, low-density electrodes were mimicked by clustering adjacent electrodes on HD-MEAs. Additionally, the AxonTracking Assay, an automated tool for recording and analyzing individual axonal arbors from many neurons in parallel, was used to characterize the function and axonal structure of recorded cultures. Results indicated that higher density and smaller electrodes provided greater sensitivity, enabling the detection of smaller spikes, and covering the full spectrum of network behavior. The high-resolution analysis of network dynamics, coupled with the AxonTracking Assay's subcellular insights, provide powerful insights into drug screening and disease modelling.