The claustrum (CLA) is a densely connected brain region that is associated with attention, motor planning, anxiety and sleep. Majority of the research studies focus on its connections to the cortex. Its connections with the subcortical areas are needed to gain more insights about its function. This study investigates the CLA focusing on its connections with the basolateral amygdala (BLA) and prefrontal brain region. Using ex-vivo patch clamp, optogenetics, and in-vivo electrophysiology, we map the BLA's inputs to the CLA and its outputs to the prefrontal cortex. Findings reveal that the CLA receives a mix of excitatory and inhibitory signals from the BLA, with prefrontal-projecting CLA neurons experiencing inhibition. The experimental measurements were used to model a spiking neural network to predict the neural activity of the prefrontal region based on the inputs of basolateral amygdala-claustrum neurons. This contributes to a deeper understanding of the neural pathways of basolateral amygdala and claustrum that will help future functional and behavioral studies.