Sleep benefits memory consolidation, especially the first hours following learning, as shown in humans and rodents. However, the effect of sex on both memory performance and its dependency on sleep remains poorly investigated. To investigate the interaction between sex and sleep on memory consolidation, we employed the Novel Object Recognition Test (NORT) in rodents, a task reliant on post-learning sleep for consolidation. Six-month-old male and female mice (n=10/group) were trained with two objects, followed by either undisturbed sleep (control group) or 5 hours of sleep deprivation (SD group). After 24 hours, one object was replaced with a novel object and animals' exploration times of each object were measured. Despite equal exploration times, only females showed a significant preference for the novel object compared to males (two-way ANOVAs, object*sex interaction, F (1, 34) = 9.104, P=.005, mean discrimination index for females= ±0.199 vs for male= ± 0.013). Furthermore, post-learning sleep deprivation only impaired female memory performance (two-way ANOVAs, object*sex interaction, F (1, 18) = 0.05661, P=.815). These findings suggest a potential sex difference in NORT performance, with females relying more on sleep for long-term memory consolidation. Further research is needed to understand the underlying mechanisms beneath the vulnerability of females to SD on NORT. This study highlights potential considerations for NORT implementation and reproducibility in memory and sleep research.