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Authors & Affiliations
Hideki Hida, Shinya Ueno, Shiori Tominaga, Yu Nishimura, Dewi Mustika, Kenta Kobayashi, Naoki Tajiri, ChaGyun Jung
Abstract
Although rehabilitative training of forced limb use (FLU) after intracerebral hemorrhagic stroke (ICH) results in forelimb functional recovery in rats, it is still unknown whether quality change of skilled reaching is induced by FLU after ICH. To analyze detail kinematics of the forelimb function, markerless pose estimation using DeepLabCut based on deep neural network was applied to ICH model rats that can be selectively inhibited in the regulatory system of motor function.To inhibit the regulatory system after functional recovery, the cerebello-rubral tract is selectively blocked with DREADD system: AAV-DJ-EF1a-DIO-hM4D(Gi)-mCherry was injected into the cerebellar lateral nucleus and FuG-E-MSCV-Cre was injected into the parvocellular red nucleus, followed by clozapine-N-oxide (CNO) administration.Detail kinematics analysis revealed that mean distance between fingertip 1 to 4 became smaller with negative correlation to success rate in non-FLU-ICH group. In addition to significant increase in the success rate, positive relationship between the mean distance and success rate was observed in FLU-ICH group. Interestingly, the blockade of the cerebello-rubral tract with CNO significantly reduced the success rate, keeping the positive correlation. Further analysis of the finger shape revealed that the quality of finger movements significantly differs between non-FLU-ICH group and FLU-ICH group.Data suggest that the cerebello-rubral tract is related to the recovery of skilled forelimb function by FLU and that quality changes of skilled reaching are also induced by rehabilitative training by FLU after stroke.