During development, the specificity of retinal neural circuit wiring is established by developmental programs that require cell adhesion molecules (CAMs) to define precise layering and connectivity. However, our current understanding of this process remains superficial, as the field has been guided primarily by gross anatomical phenotypes that can be visually quantified. Here, we present an alternative approach to studying circuit wiring that focuses on functional changes rather than relying on morphological ones. To this end, we use the mouse retina as a model and employ a recently developed large-scale retinal ganglion cell (GC) imaging approach that allows functional analysis of up to 40% of the surface area of a single retina. In our project, we focus on the role of adhesion proteins responsible for synaptic wiring between the two key components of the direction-selective circuit: starburst amacrine cells (SACs) and direction-selective GCs. First, we characterized CAMs using cell surface proteomics of the pan-GCs and SACs "surfaceome" and defined candidates based on protein-network interactions. Second, we are working to establish a postnatal (P0-P2), cell-type-specific CRISPR-mediated approach that allows specific disruption of candidate CAMs in SACs while functionally assessing large-scale GC responses. In this poster, we will show some preliminary data on full knockout assessment of some candidate proteins.