Hearing losses are the most prevalent sensory disorder. The hearing organ, the organ of Corti, is located in a bony capsule in the pars petrosa of the temporal bone. Its spiralling morphology makes it difficult to investigate the cellular mechanism of hearing. The cells provide morphological and physiological differences, called tonotopy, which is the base of the frequency selectivity. The supporting cells have similar variability to the receptor cells, however they receive little attention. One type of the supporting cells are the Deiters cells which are in close connection with the outer hair cells, and therefore may influence their development, regeneration, and the hearing threshold. Mathematical models could replace the difficult preparation, but there are no models for the supporting cells. Our aim was to set up a mathematical model for the Deiters cells, which could reliably describe their calcium handling (induced by purinergic receptors) mechanism considering tonotopy. To validate our model, we used functional calcium imaging recordings of single cell dyed Deiters cells from mice (day 15, BALB/c, n=75, turns: basal, middle, apical, induced with 100 μM ATP). The differences of the tonotopic regions observed during the experiments (amplitude, duration of the response, area under curve) could be implemented with fitting of different parameter sets. To find the best model we tested more than 5 million parameter sets. Our mathematical model is suitable for predicting the calcium transients of the Deiters cells in each turn and may help the development of this research area.