During Gaze stabilization extraocular muscles produce reflexive compensatory eye movements to fix the image on the retina. Among such a complex sensorimotor transformation the vestibulo-ocular reflexes (VOR), generating compensatory eye movements in response to head motion, require vestibular inputs which are integrated by extraocular motoneurons.In Xenopus larvae, the onset of the angular VOR (aVOR) is delayed compare to other visuo-vestibular reflexes. This delay could imply the existence of distinct parallel pathways in vestibulo-ocular networks, involving distinct motoneuron functional subpopulations.Although relevant for understanding the establishment of these parallel pathways, the maturation processes of abducens motoneurons have not been elucidated. This led us to investigate how their properties differ during the establishment of the aVOR during pre-metamorphosis life. Firstly, we performed electrophysiological recordings of unitary nerve discharges on in vitro isolated head preparations during passive head rotations. The abducens nerve discharge analysis, based on their spike amplitude, revealed different motor unit groups with distinct maturation patterns from the aVOR onset (stage 49-50) to a more mature stage (stage55-56). These results will be complemented with immunohistochemistry (Kv1.1, Kv3.1b, Cav3.3) and electron microscopy experiments, investigating the molecular phenotypes the axon myelination and neuronal size between these two stages.In the future, we intend to perform the same approach on other extraocular motor nuclei, involved in ocular reflexes mature earlier, and in which we suspect different developmental patterns. These findings enhance our understanding of the development in neural networks controlling gaze-stabilizing ocular behavior in vertebrates.