Our previous studies have suggested that acupuncture modulates the mesolimbic dopamine(DA) system, a region implicated in drug abuse. Therefore, in this study, we hypothesized that the stimulation of acupuncture activates mesolimbic GABA neurons in the ventral tegmental area(VTA), thereby affecting the inhibition of addictive behavior. Cocaine injections and LI5 acupuncture stimulation significantly increased locomotor activity, but HT7 acupuncture inhibited cocaine-induced locomotion. In vivo fiber photometry showed that HT7 stimulation significantly activated VTA GABA neurons compared to controls. After stimulating HT7 in mice and creating acute brain slices, HFS did not alter the excitatory postsynaptic potential(EPSC) in VTA GABA neurons compared to controls. However, the control group showed a significant increase in the paired-pulse ratio(PPR) post-stimulation, which was absent in the HT7 group. This suggests that LTP had already been established in VTA GABA neurons by HT7 stimulation, thereby occluding the effects of HFS. These findings suggest that acupuncture at the HT7 activates GABA neurons within the VTA, mitigating cocaine-induced locomotion through mechanisms possibly involving neural plasticity, specifically LTP formation. This study contributes novel insights into the mechanisms through which acupuncture may serve as a promising alternative therapy for drug addiction, highlighting its potential to modulate addictive behaviors through neurobiological pathways.Funding: National Research Foundation of Korea(NRF) Grants funded by the Korea government(MSIT) 2018R1A5A2025272,2020R1A2C1103154 RS-2023-00262398 and Korea Medical Device Development Fund grant funded by the Korea government(the Ministry of Science and ICT, the Ministry of Trade, Industry and Energy, the Ministry of Health&Welfare, the Ministry of Food and Drug Safety) RS-2023-00253560.