In adult mouse visual cortex, neuronal responses to visual stimuli undergo gradual and continuous changes over time. This phenomenon called “representational drift” has also been observed in other sensory and higher cortical areas and has profound implications for coding strategies in the brain.It is unknown which tuning feature changes of individual neurons underlie representational drift in the primary visual cortex (V1), and what regulates their drift. Using repeated 2-photon calcium imaging in awake mice, we show a slow, time-dependent drift of the preferred orientation of V1 neurons over days to weeks. In olfactory cortex it has been shown that the repeated experience of an odor stabilizes its neuronal representations. We tested whether a similar effect occurs in the visual system by mounting cylinder lens goggles in front of the mice’s eyes for several weeks, thus limiting visual experience to a narrow range of orientations. We found that this intervention did not alter drift magnitude but instead single-cell orientation preference drifted towards the experienced orientation, suggesting that the statistics of the visual environment steers the direction of drift. Furthermore, we started investigating the role of local circuit inhibition using sustained chemogenetic reduction of parvalbumin-positive interneuron activity. Preliminary results indicate that lowering inhibition increases drift.Together, our results demonstrate that the preferred orientation of individual neurons in the visual cortex undergoes representational drift and that the statistics of the visual environment determine the direction of this drift.