Antidepressant treatment during pregnancy may endanger neurodevelopment of the offspring. Cellular parameters (hippocampal homeostasis) and physiological parameters (behavioral aspects) in the offspring of Mirtazapine treated and depressed mothers are not yet fully described. We evaluated changes in cellular calcium homeostasis (Fura2AM) and CaV1.2 expression, presence of mitochondrial superoxide radicals (MitoSOXTM) together with behavior of developing pups.As an experimental model of perinatal depression we used pre-gestational exposure to chronic unpredictable stress followed by Mirtazapine treatment at a clinically relevant dose of 10 mg/kg/day. For cellular analyses primary hippocampal cultures at DIV13 were used. Behavioral tests were performed at postnatal days 6-12.Density of CaV1.2 channels was upregulated in stressed and Mirtazapine treated rats. Amount of present superoxide radicals was increased, as well. Righting reflex exhibited significant slowdown. This could indicate a possible slowing of the maturation of motor reflexes.Taken together our results demonstrate considerable changes in stressed and Mirtazapine treated groups, therefore we conclude Mirtazapine as an antidepressant is affecting cellular homeostasis and physiological development of the pups.Supported by grant APVV-19-0435