During cortical development, intrinsic and extrinsic factors regulate how radial glial cells (RGs) proliferate and produce neurons in a highly controlled manner. To date, investigation of the mechanisms regulating radial migration has predominantly relied on studies of cultured cortical slices or employed in utero electroporation strategies. However, these methods are not suitable for investigating the role of gradients of extracellular signals. To address this, we developed an innovative in vitro model where RGs processes support the migration of young pyramidal neurons generated in situ.We place primary mouse RGs cultures on patterned coverslips and differentiate only a subset of the aligned RGs into post-mitotic neurons. Using time-lapse video microscopy, we characterize the migratory behaviour of these neurons as they navigate along the RGs scaffold, revealing a stereotypical saltatory migration pattern and parameters that are consistent with previous ex vivo studies. To observe cell migration in response to extracellular factors, we used the Dunn chamber, a microfluidic device capable of generating linear, long-lasting gradients. We are currently validating our new in vitro model by testing chemotropic molecules; the effects of which have been thoroughly described in the context of radial migration.Using our new model, we aim to identify novel extracellular regulators of radial migration and elucidate the underlying subcellular mechanisms. By overcoming the limitations of previous methods, our approach promises to advance our understanding of neuronal migration processes.