Neonatal ischemic stroke is a significant cause of mortality, severe developmental delays, and spastic cerebral palsy. In most cases, ischemic stroke occurs in the basin of the middle cerebral artery. Middle cerebral artery occlusion is the well-established model for stroke in adults. However, this method is mostly described in rat pups and is not well-established in neonatal mice.In this study, we compared a model of neonatal stroke (tfMCAO, transient filament middle cerebral artery occlusion) with neonatal hypoxic-ischemic brain injury (RVM, Rice-Vannucci model of unilateral carotid ligation and 1.5h of hypoxia). Stroke was induced in postnatal day 7 or 9 mice and sacrificed in time frames 4 or 6h after the procedure to determine the appropriate model. Infarction was validated by TTC and cresyl violet staining. Our findings indicated that the most reproducible focal infarction with lesser mortality rate was at 4h postnatal day 7 mice with stroke induced by the tfMCAo. By immunostaining, we obtained an expression profile of the cell markers in the ischemic core, penumbral, and intact areas. Noticeably in the penumbral area, we observed positive progenitor cell markers, that could give more insight into the functional process of the injured penumbral cells.Our study demonstrates that tfMCAo model is a reproducible ischemic stroke model in neonatal mice, further, we can compare it to adult stroke. Moreover, the identification of the positive cell markers in the penumbral area will be valuable for investigating the function of dynamics, response to injury, and self-regeneration potential within the neonatal brain