The dorsal tegmentum including the ventrolateral PAG (vlPAG) and dorsal raphe nucleus (DRN) plays a critical role in controlling anxiety, fear memory formation, autonomic processes and most particularly, it is involved in descending modulation of pain processing. It has been shown that different neuron types, such as dopaminergic and serotonergic cells are part of this circuitry – besides the glutamatergic and GABAergic neurons – however their functions remained unclear. Here, we explored the neuronal activity in the vlPAG and DRN in urethane-anesthetized mice in response to different types of sensory stimuli by applying two different recording approaches. First, we performed acute silicone probe recordings to determine how neurons respond to different types of stimulation. We observed that neurons in the dorsal tegmentum responded distinctly to sensory stimulations. Second, using the juxtacellular recording technique with the same set of external stimuli and post hoc immunocytochemistry to identify the recorded cell types, we distinguished neurochemically different neuron types in this region. We found that dopaminergic neurons were typically excited by foot shocks and could be separated into two groups based on their response latency and vasoactive intestinal polypeptide content, suggesting their different involvement in noxious stimulation processing. Further, we revealed that serotonergic neurons were heterogeneous and could be clustered into five groups based on their responses upon noxious stimulation. Our current results show that the firing of the monoaminergic neurons in the dosal tegmentum circuitries are distinctly modified by noxious stimuli, implicating their different contribution to pain processing in this clinically important brain region.