PhospholipaseC γ2 (PLCG2) is known to have direct link with genetic risk factors for Alzheimer’s like dementia (AD). Previous work showed its action in AD is regulated through microglia. However, the synaptic/ electrophysiological correlates are elusive. In present study, WT mouse were transfected with sh-RNA of LV-PLCG2 (U6 promotor, GFP as reporter; shRNA) or scrambled (used as control; shNT). Whole-cell voltage-clamp recordings were performed on acute slices with glass pipette (<3-4MΩ) at -70mv, followed by morphometry. Two-tailed Mann-Whitney test was used for statistics unless otherwise mentioned, p<0.05 was considered to be significant. mEPSC amplitude was found to be reduced (p=0.0288) in shRNA condition, even the cumulative probability of mEPSC amplitude was also found to be significantly altered after silencing of PLCG2 gene in DG cells (p=0.0014; Kolmogorof-Smirnov test). mEPSC frequency of the miniature activity of transfected DG cells also found to be significantly reduced (p=0.002). Sholl analysis of the DG cells expressing shRNA showed a decrease in the number of intersections vs distance from soma (P<0.05), accompanied by significant decrease in dendritic length (p=0.0501), dendritic volume (p<0.0001) and dendritic surface area (p=0.0273). When spine density was measured we have found a significant decrease in cumulative distribution of spine density (p<0.0001; Kolmogorof-Smirnov test), which is tightly associated with decrease in spine length (p=0.0209) and spine head diameter (P<0.0001). Furthermore, these dendritic and spine morphometric alteration is also accompanied with the decrease in the volume of mossy fiber gigantic bouton (mfB) with alteration in the number of filopodia per mfBs (p<0.05).