We have recently identified Tusc5 as a Retinal Ganglion Cell (RGC) marker, specifically expressed in Brn3a+ RGCs, and absent from Brn3aKO retinas. Tusc5 is a transmembrane protein that assists trafficking of Glucose transporter GluT4 in adipocites. GluT4 are continuously recycled through endosomes from and to the plasma membrane and stored in GSVs (Glut4 Storage Vesicles), in an insulin-dependent manner. In adipocites, GluT4 recycling is assisted by Tusc5. In mouse retina, Tusc5 is expressed specifically in a subset of Brn3a+ retinal ganglion cells (RGCs), characterized by small dense “bushy” dendritic arbors laminating between the characteristic ON and OFF ChAT-positive bands of the inner plexiform layer (IPL), and by ‘bursty’ unsustained responses to the light stimulation with strong surround suppression of the receptive field. To better study Tusc5 function, we generated a Cre-dependent, conditional Tusc5 knock-in eGFP reporter mouse allele (Tusc5CKOeGFP). By crossing our allele with a mouse strain expressing Cre in the germline, we generated a full body knock-in reporter line (Tusc5EGFP). eGFP faithfully coincides with Tusc5 expression in RGCs from Tusc5eGFP/WT (heterozygote) mice, and the Tusc5 protein is absent from Tusc5eGFP/eGFP (knockout) mice. We use Tusc5EGFP mice to characterize Tusc5+ RGCs and their electrophysiological responses, and survey neuronal expression of Tusc5. Using immunofluorescence experiments in retinas from Tusc5eGFP/WT mice and HEK293 cells transfected with Tusc5-HA constructs we surveyed the cellular compartments expressing Tusc5. Our results suggest a role for Tusc5 in energy metabolism in neurons.Funding: Unitatea Executiva pentru Finantarea Invatamantului Superior, a Cercetarii, Dezvoltarii si Inovarii Grant PN-III-P4-PCE-2021-0333