The study of rodent navigation has for decades focused on the hippocampal and parahippocampal regions, although recently part of this focus has shifted towards whether positional information can be identified in lower association cortices or even primary sensory areas. Spatial representation in these regions is often investigated using one-dimensional paradigms in combination with virtual reality. In the present work, we seek to understand how position is encoded in a more naturalistic, two-dimensional environment. Starting at the sensory level and moving up to higher association areas we aim to see how these regions differ and relate to one another, both on a cell-to-cell basis as well as on the population level.Using a miniature portable two-photon microscope (MINI2P) for freely moving mice, as well as Neuropixels 2.0 probes (in separate animals), we have investigated hundreds of spatially tuned cells in the adult mouse primary visual cortex, retrosplenial cortex, medial entorhinal cortex and the hippocampus. The standard experimental set-up involved free foraging for treats in an 80cm x 80cm open field arena. As interaction with objects/landmarks is an essential part of navigation, a second set of experiments involved introducing a visually salient tower to the arena, which was then moved and removed in subsequent sessions.Our data shows the presence of clear spatial firing patterns, both in the presence and absence of objects, in the visual and retrosplenial cortex that might elucidate the computations involved in the creation of positional representation in the hippocampal and parahippocampal formation.