Spinocerebellar ataxia type 1 (SCA1) is an autosomal dominant neurodegenerative disease manifested by cerebellar disorders. Alongside cerebellar symptoms, individuals with SCA1 often exhibit a range of ophthalmic abnormalities, particularly those affecting the optic nerve and retina. However, the mechanisms underlying visual disturbances in SCA1 remain unclear.Therefore, in this study, we aimed to analyze the retinal cell layers of mice with SCA1.Sixteen eye samples were obtained from 6-month-old mice (8 from SCA1 mice and 8 from wild-type). The volume of the retina, as well as the volume and number of cells in the outer and inner nuclear and ganglionic layers, were determined on equidistant paraffin sections stained with hematoxylin and eosin using unbiased stereology approaches. Antibodies to rhodopsin [1D4] were used to estimate the number of rod nuclei in the outer nuclear layer.After processing the results, we realized that there were no statistically significant differences between the general retinal volume, the volume, number of cells, and cell density in the outer and inner nuclear and ganglion layers of wild-type and SCA1 mice.The results obtained from the morphological analysis did not confirm the assumption regarding changes in the retina of SCA1 mice at the age of 6 months. Our findings indicate that retinal changes are not evident at the histological level in six-month-old mice but necessitate further study in older age groups. This study was supported by EMBO, grant number SLG 5433 and by the Cooperatio Program, research area MED/DIAG.