Deep Brain Stimulation (DBS) of the hippocampal formation is currently investigated as a therapeutic approach for the treatment of memory disorders and epilepsy. Although various neurostimulation protocols have been extensively studied semi-acutely in epileptic patients, their refinement requires more standardized preclinical testing in non-human primates (NHP). However, targeting deep brain structures such as the hippocampal formation in NHP still remains a challenging task. Here, we present a technological framework for submillimeter targeting of the hippocampal formation in macaque monkeys using miniaturized DBS electrodes and a multi-camera optical neuronavigation system guided by Magnetic Resonance Imaging (MRI) and Computed Tomography (CT) data. We demonstrate the accuracy of our approach in phantom models and in macaques implanted with two DBS electrodes targeting the hippocampus and entorhinal area.Specifically, surgical planning was based on high-resolution (0.5 mm isotropic) T1- and T2-weighted MRI images and 150-um CT scans, co-registered to determine each trajectory’s entry point and target. Additionally, high-resolution diffusion MRI acquisitions at an isotropic resolution of 0.7 mm enabled to visualize the precise location of white matter fiber tracts such as the fornix with respect to the planned trajectories. Surgical planning and neuronavigation were performed using a 6-camera neuronavigation system (Cortexplore). A custom procedure was designed to implant custom 8-contact DBS electrodes (Dixi Fablab) at the targeted locations and connect them to a commercially available neurophysiology system (Blackrock Neurotech), including a dedicated electrode holder and custom percutaneous connector. Future experiments will investigate the impact of hippocampal DBS on learning and memory in NHPs.