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AGE-DEPENDENT CHANGES IN SYNAPTIC PLASTICITY AT PARALLEL FIBER–PURKINJE CELL SYNAPSES IN NORMAL AGED MICE
Ritsuko Inoueand 1 co-author
Tokyo Metropolitan Institute for Geriatrics and Gerontology
FENS Forum 2026 (2026)
Barcelona, Spain
Board PS06-09PM-278
Presentation
Date TBA
Board: PS06-09PM-278
Event Information
Poster Board
PS06-09PM-278
Poster
View posterAbstract
Age-related decline in brain function has been observed across multiple brain regions. Accordingly, neuronal circuit functions, including synaptic plasticity, are also thought to be affected by aging. However, how the progression of aging influences synaptic plasticity remains largely unclear.
Long-term depression (LTD) at the parallel fiber (PF)–Purkinje cell (PC) synapse in the cerebellar cortex (PF-LTD) is considered a cellular substrate for cerebellum-dependent motor learning, including delay eyeblink conditioning (dEBC). At the same PF synapse, we have previously shown that long-term potentiation (PF-LTP) is markedly reduced in cerebellar slices obtained from mice older than 20 months of age. In addition, dEBC performance is significantly impaired in 85–90-week-old (approximately 20-month-old) C57BL/6J mice. Based on these findings, it is reasonable to hypothesize that PF-LTD at the same synapse is also diminished in aged mice. However, the effects of aging on PF-LTD remain poorly understood, as only a limited number of studies using 12-month-old CBA mice have addressed this issue to date.
Therefore, we performed whole-cell patch-clamp recordings from Purkinje cells in acute cerebellar slices obtained from C57BL/6J mice across three age groups—young adult (3–4 months), middle-aged (12–18 months), and aged (20–24 months)—to examine age-dependent changes in PF-LTD and to compare the effects of aging on PF-LTD with those on PF-LTP.
Long-term depression (LTD) at the parallel fiber (PF)–Purkinje cell (PC) synapse in the cerebellar cortex (PF-LTD) is considered a cellular substrate for cerebellum-dependent motor learning, including delay eyeblink conditioning (dEBC). At the same PF synapse, we have previously shown that long-term potentiation (PF-LTP) is markedly reduced in cerebellar slices obtained from mice older than 20 months of age. In addition, dEBC performance is significantly impaired in 85–90-week-old (approximately 20-month-old) C57BL/6J mice. Based on these findings, it is reasonable to hypothesize that PF-LTD at the same synapse is also diminished in aged mice. However, the effects of aging on PF-LTD remain poorly understood, as only a limited number of studies using 12-month-old CBA mice have addressed this issue to date.
Therefore, we performed whole-cell patch-clamp recordings from Purkinje cells in acute cerebellar slices obtained from C57BL/6J mice across three age groups—young adult (3–4 months), middle-aged (12–18 months), and aged (20–24 months)—to examine age-dependent changes in PF-LTD and to compare the effects of aging on PF-LTD with those on PF-LTP.
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