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ANATOMICAL INVESTIGATION OF REGIONS GENERATING SPONTANEOUS POPULATION ACTIVITY IN THE HUMAN EPILEPTIC HIPPOCAMPAL FORMATION
Nour Essamand 6 co-authors
HUN-REN ttk Research Centre for Natural Sciences, Pázmány Péter Catholic University, Faculty of Information Technology and Bionics
FENS Forum 2026 (2026)
Barcelona, Spain
Board PS05-09AM-382
Presentation
Date TBA
Board: PS05-09AM-382
Event Information
Poster Board
PS05-09AM-382
Poster
View posterAbstract
Mesial temporal lobe epilepsy – a neurological disorder with frequent pharmacoresistant cases – is characterized by unpredictable seizures. The hippocampal formation is closely associated with seizures generation. In this project we investigated postoperative hippocampal tissue slices derived from human epileptic patients. We examined the CA2 and the subiculum regions, in vitro, using electrophysiological and anatomical techniques. The local field potential gradient was recorded with a 24-channel linear microelectrode covering all layers of the examined regions. Spontaneous population activity (SPA) resembling interictal-like activity was detected in both regions (subiculum n= 11/16 slices, CA2 n= 7/9 slices). Higher neuronal firing was observed in the CA2 region, than in the subiculum during the SPA events. Slices either generating or lacking SPA were identified and chosen for further light and electron microscopic investigation. NeuN-staining was used to measure neuronal density, and Ca2+-binding protein calbindin (CB) was used for measuring the synaptic input of neurons at electron microscopic level. CA2 and subicular neuronal somata received predominantly inhibitory synaptic input, but excitatory synapses were also observed. Morphology of CB+ cells did not show differences between SPA and no-SPA regions. The presence of perforated synapses suggests synaptic reorganization in the human epileptic hippocampus. We identified calbindin-positive inhibitory axon terminals forming synaptic contacts on the soma of pyramidal neurons suggesting the presence of a calbindin-positive basket cell type in the human epileptic hippocampal formation.
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