Poster preview
ePoster
DIFFERENTIAL EFFECTS OF OUABAIN ON BDNF EXPRESSION IN LUNG AND BRAIN IN A MURINE MODEL OF MIXED ASTHMA
Martina Ribeiroand 11 co-authors
University of São Paulo
FENS Forum 2026 (2026)
Barcelona, Spain
Board PS06-09PM-284
Presentation
Date TBA
Board: PS06-09PM-284
Event Information
Poster Board
PS06-09PM-284
Poster
View posterAbstract
Brain-derived neurotrophic factor(BDNF) is a neurotrophin involved in neural plasticity and has been implicated in eosinophilic asthma, but its role in mixed asthma remains unclear. Mixed asthma is a chronic inflammatory condition characterized by the combined involvement of eosinophils and neutrophils. Ouabain(OUA), a Na⁺,K⁺-ATPase inhibitor, exhibits neuromodulatory and immunomodulatory effects and reduces allergic airway inflammation. Here, we investigated the effects of OUA on BDNF expression in a murine model of mixed asthma.
Methods: Female BALB/c mice were sensitized with ovalbumin(OVA) and Complete Freund’s Adjuvant and challenged intranasally with OVA on days21–23. OUA(0.56mg/kg, i.p.) was administered 1h before each challenge. Experiments were performed on day 24. Lung inflammation was assessed by bronchoalveolar lavage(BAL) cell counts, and BDNF levels were quantified by ELISA. Data were analyzed using one-way ANOVA.
Results: BAL fluid from OVA-challenged mice showed a significant increase in total cells (n=5)(×10⁴/mL:116±12P<0.0001), neutrophils (54±4,P<0.0001), and eosinophils (59±8,P<0.0001). Total lung cell recruitment was also increased. In lung tissue, BDNF levels (pg/mL) were elevated in groups treated with ouabain: OUAvsPBS (312±203,P<0.0003) and OVAvsOVA+OUA (274.8±617.6,P<0.0001)(N=7). In the frontal cortex, OUA treatment decreased BDNF levels in the OVA+OUA group compared to PBS (313±575,P<0.02)(N=6).
This mixed asthma reflects the main pathological features described in the literature. OUA treatment significantly increased BDNF levels in the lungs of asthmatic and non-asthmatic animals. Our preliminary results indicate that OUA treatment in asthmatic animals reduced BDNF expression in the frontal cortex, with no changes observed in the hippocampus.Recommended posters
DIFFERENTIAL EFFECTS OF OUABAIN ON NA⁺,K⁺-ATPASE FUNCTION: A KINETIC AND ACTIVITY STUDY
Eka Nozadze, Lia Shioshvili, Nana Arutinova, Marine Leladze, Sopio Dzneladze, Maia Javakhishvili, Leila Tsakadze, Nino Metreveli, Gvantsa Chkadua
BDNF/PROBDNF SIGNALING REGULATES MICROGLIAL PHENOTYPE AND INFLAMMATORY RESPONSES IN THE MOUSE HIPPOCAMPUS
Alexandra Theurer, Yesheng Sun, Martin Korte, Marta Zagrebelsky
CIRCULATING BDNF IS ASSOCIATED WITH ATTENUATED INFLAMMATION-RELATED NEUROAXONAL INJURY WHILE TAU-RELATED BIOMARKERS REMAIN ADDITIVE
Yue Shi, Takuji Kawamura, Kentaro Oba, Yasuyuki Taki
SSAO-MEDIATED DECREASE OF ENDOTHELIAL BDNF RELEASE AFFECTS NEURONAL GLUA1 AND PSD95 EXPRESSION: A PERIPHERAL MECHANISM INDUCING NVU AND CNS DISTURBANCES
Alfredo J. Miñano-Molina, Cristina Fábregas-Ordóñez, Dolores Siedlecki-Wullich, Rut Fadó, Núria Casals, Mercè Unzeta, Jose Rodríguez-Álvarez, Montse Solé
NEUROPROTECTIVE EFFECTS OF VEGF-B IN A MODEL OF SELECTIVE AND LATE NEURONAL DEATH
Yoan Reynaldo Torres Cruz, Eva M. Baranda-Alonso, Pablo G. Téllez de Meneses, Naiara Martín-Llorente Hamed, Ericka Elescano-Delgado, Jimena Pérez Arévalo, Carla Escudero-Solano, David Díaz, Eduardo Weruaga
A NOVEL DISTAL ENHANCER REGION INVOLVED IN THE REGULATION OF BRAIN-DERIVED NEUROTROPHIC FACTOR EXPRESSION
Liis Kuusemets, Eli-Eelika Esvald, Jürgen Tuvikene, Olga Jasnovidova, Tõnis Timmusk