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EXACERBATED HIPPOCAMPAL NEUROINFLAMMATION IN FEMALE CD-1 MICE UNDER HYPERCALORIC DIET AND ALLOXAN-INDUCED DIABETES
Alma Karen Lomeli-Lepeand 3 co-authors
University of Guadalajara
FENS Forum 2026 (2026)
Barcelona, Spain
Board PS03-08AM-478
Presentation
Date TBA
Board: PS03-08AM-478
Event Information
Poster Board
PS03-08AM-478
Poster
View posterAbstract
We investigated hippocampal glial responses in a combined dietary and pharmacological murine model of diabetes using female CD-1 mice. Animals (n = 5 per group) were maintained on a hypercaloric diet (HCD) for 4 weeks starting at ~30 g body weight; one group then received alloxan while continuing HCD for an additional week (HCD+ALLOXAN) prior to sacrifice. Immunofluorescence against Iba-1 (microglia) and GFAP (astrocytes) was performed on hippocampal sections and optical density (OD) quantified in the dentate gyrus (DG), CA1 and CA3 regions. Compared with control mice, HCD induced significant increases in both GFAP and Iba-1 OD across all hippocampal subregions, indicating enhanced astrocytic and microglial reactivity under metabolic challenge. Importantly, the addition of alloxan further elevated GFAP and Iba-1 signals relative to HCD alone in every region analyzed, suggesting that alloxan-induced β-cell toxicity exacerbates hippocampal neuroinflammatory responses beyond the effects of diet alone. These findings demonstrate that a hypercaloric diet elicits widespread glial activation in the female CD-1 hippocampus and that this effect is amplified by alloxan administration. The combined HCD+ALLOXAN model thus provides a robust platform for studying diabetes-associated neuroinflammation in key hippocampal circuits relevant to cognition and metabolic dysfunction.
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