Poster preview
ePoster
GENOTYPE‑DEPENDENT REGULATION OF ATTP AND CALCIUM‑BINDING INTERNEURONS IN CA1 STRATUM ORIENS DURING AGING AND A‑TOCOPHEROL SUPPLEMENTATION
Jorge Selva-Clementeand 4 co-authors
Human Neuroanatomy Laboratory (HNL), Institute of Biomedicine (IB) and Instituto de Investigación Sanitaria de Castilla-La Mancha (IDISCAM), School of Medicine, University of Castilla-La Mancha (UCLM)
FENS Forum 2026 (2026)
Barcelona, Spain
Board PS01-07AM-072
Presentation
Date TBA
Board: PS01-07AM-072
Event Information
Poster Board
PS01-07AM-072
Poster
View posterAbstract
Age‑related neurodegeneration involves oxidative stress and calcium imbalance, making the hippocampal CA1 region particularly vulnerable. α‑Tocopherol (αT), a major antioxidant, depends on α‑Tocopherol Transfer Protein (αTTP) for intracellular handling, yet how αTTP‑expressing cells and calcium‑binding interneurons respond to aging and antioxidant treatment is not well understood.
This study examined αTTP‑immunoreactive (IR) cells and their colocalization with calbindin (CB), parvalbumin (PV), and calretinin (CR) interneurons in the CA1 stratum oriens of SAMP8 (accelerated aging) and SAMR1 (control) mice, with and without αT supplementation.
αTTP‑IR density remained stable with age in SAMR1 but increased markedly in SAMP8, revealing a strong genotype–age interaction. αT treatment further elevated αTTP‑IR density in SAMP8 at both 6 and 12 months but had no effect in SAMR1. CB‑IR density showed no age‑related changes, though SAMR1 displayed higher values than SAMP8 at early ages; αT supplementation increased CB‑IR only in young SAMP8. PV‑IR density declined with age in SAMR1 but increased progressively in SAMP8, unaffected by αT supplementation. CR‑IR density was largely stable, except for an αT‑induced increase in aged SAMR1.
Colocalization patterns showed limited aging effects: PV/αTTP colocalization was consistently higher in SAMR1, while CR/αTTP colocalization increased with age in αT‑treated SAMP8.
Overall, αTTP‑expressing cells and specific interneuron subtypes respond differently to genotype, aging, and αT supplementation, highlighting αTTP‑related mechanisms as potential targets for antioxidant-based interventions in hippocampal aging.
Financially supported by 2022-GRIN-34392 and 2025-GRIN-38527 UCLM
This study examined αTTP‑immunoreactive (IR) cells and their colocalization with calbindin (CB), parvalbumin (PV), and calretinin (CR) interneurons in the CA1 stratum oriens of SAMP8 (accelerated aging) and SAMR1 (control) mice, with and without αT supplementation.
αTTP‑IR density remained stable with age in SAMR1 but increased markedly in SAMP8, revealing a strong genotype–age interaction. αT treatment further elevated αTTP‑IR density in SAMP8 at both 6 and 12 months but had no effect in SAMR1. CB‑IR density showed no age‑related changes, though SAMR1 displayed higher values than SAMP8 at early ages; αT supplementation increased CB‑IR only in young SAMP8. PV‑IR density declined with age in SAMR1 but increased progressively in SAMP8, unaffected by αT supplementation. CR‑IR density was largely stable, except for an αT‑induced increase in aged SAMR1.
Colocalization patterns showed limited aging effects: PV/αTTP colocalization was consistently higher in SAMR1, while CR/αTTP colocalization increased with age in αT‑treated SAMP8.
Overall, αTTP‑expressing cells and specific interneuron subtypes respond differently to genotype, aging, and αT supplementation, highlighting αTTP‑related mechanisms as potential targets for antioxidant-based interventions in hippocampal aging.
Financially supported by 2022-GRIN-34392 and 2025-GRIN-38527 UCLM
Recommended posters
NEURONAL RTP801 IMPAIRS ADULT HIPPOCAMPAL NEUROGENESIS VIA DYSREGULATED NON–CELL-AUTONOMOUS SIGNALING IN ALZHEIMER’S DISEASE
Pol Garcia-Segura, Almudena Chicote-González, Marta Garcia-Alcaraz, Júlia Solana-Balaguer, Genís Campoy-Campos, Lea Michalke, Laura Ledo-Sainz, Eric Romera-Carvajal, Maria Lázaro-Clos, Joaquín Fernández-Irigoyen, Enrique Santamaría, Manuel J. Rodríguez, Albert Giralt, Jordi Alberch, Cristina Malagelada
RECEPTOR PROTEIN TYROSINE PHOSPHATASE (RPTP) Β/Ζ MODULATES PERINEURONAL NETS AND PARVALBUMIN INTERNEURONS IN THE HIPPOCAMPUS OF APP/PS1 MICE
Elisa Rivera Illades, Teresa Fontán-Baselga, María Del Pilar Ramos-Álvarez, Gonzalo Herradón, Marta Vicente-Rodríguez, Esther Gramage
CA3 HYPERACTIVITY DRIVES HIPPOCAMPAL CA1 NEURONAL DYSFUNCTION IN AGING
Manuel Dias-Silva, Aurore Ribera, Paula A. Pousinha, Luísa V. Lopes
CHANGES IN THE POPULATION OF PARVALBUMIN-EXPRESSING INTERNEURONS IN THE HUMAN HIPPOCAMPAL FORMATION IN ALZHEIMER'S DISEASE: A ROSTROCAUDAL STUDY
Esther Buendía, Antonio Palencia, Noemí Villaseca, Jorge Selva, Mercedes Íñiguez de Onzoño, Alicia Vela, Ana María Insausti, Paul Yushkevich, Lisa Levorse, Amanda Denning, Ranjit Ittyerah, David Irwin, Ricardo Insausti, María del Mar Arroyo-Jimenez, Pilar Marcos Rabal
TARGETING INTRACELLULAR A-ΒETA OLIGOMERS PROMOTES NEUROGENESIS-DEPENDENT RESCUE OF HIPPOCAMPAL FUNCTION AND MEMORY IN AN ALZHEIMER'S DISEASE MOUSE MODEL
Laura Coppola, Raffaella Scardigli, Elena Fiori, Sofia Mancini, Silvia Middei, Federico La Regina, Giovanni Meli, Antonino Cattaneo
ROLE OF NATURAL ANTIOXIDANTS ON TISSUE TRANSGLUTAMINASE LEVELS IN GLIAL OLFACTORY CELLS EXPOSED TO AMYLOID-Β: INTEGRATED BIOCHEMICAL AND COMPUTATIONAL STUDY
Rosalia Maria Pellitteri, Cristina Tomasella, Maria Assunta Chiacchio, Matteo Pappalardo, Michela Spatuzza, Maria Vincenza Catania, Salvatore Guccione, Agatina Campisi