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INVESTIGATING THE MECHANISMS OF ACTION OF INTRAVENOUS STEM CELL THERAPY FOLLOWING TRAUMATIC SPINAL CORD INJURY
Tamás Bellákand 4 co-authors
University of Szeged
FENS Forum 2026 (2026)
Barcelona, Spain
Board PS01-07AM-437
Presentation
Date TBA
Board: PS01-07AM-437
Event Information
Poster Board
PS01-07AM-437
Poster
View posterAbstract
Traumatic spinal cord injury (SCI) is initiated by an acute mechanical insult, followed by secondary processes. In this study, we investigated the effects of delayed intravenous admin-istration of neuroectodermal (NE-GFP-4C) stem cells on reducing secondary injury, promot-ing tissue preservation, and enhancing functional recovery.
Adult female Sprague-Dawley rats received a moderately severe thoracic (T5) contusion injury, followed by intravenous stem cell infusion at 30 minutes, 1, 2, or 3 weeks post-injury. Control animals underwent injury without cell treatment. Functional outcomes (BBB scoring, kinematic analysis) and detailed morphological analyses (retrograde labelling, im-munohistochemistry) were performed, and cytokine expression in the spinal cord, spleen, and blood serum was assessed one day after cell administration using proteome profiler ar-rays.
Significant functional improvement and neuroprotection were observed in animals treated immediately or one week after injury, with increased numbers of retrogradely labelled su-pra- and propriospinal neurons compared with controls. Early and one-week-delayed stem cell infusion elevated TIMP-1 levels in the injured spinal cord and spleen, and immediate treatment altered splenic cytokine expression, highlighting the spleen as a key target of stem cell effects.
Our findings indicate that immediate or one-week-delayed intravenous stem cell therapy induces morphological and functional recovery through TIMP-1 modulation.
Funding: This work was supported by Albert Szent-Györgyi Grant provided by the Albert Szent-Györgyi Medical School, University of Szeged to KP (SZGYA-KKA).
Adult female Sprague-Dawley rats received a moderately severe thoracic (T5) contusion injury, followed by intravenous stem cell infusion at 30 minutes, 1, 2, or 3 weeks post-injury. Control animals underwent injury without cell treatment. Functional outcomes (BBB scoring, kinematic analysis) and detailed morphological analyses (retrograde labelling, im-munohistochemistry) were performed, and cytokine expression in the spinal cord, spleen, and blood serum was assessed one day after cell administration using proteome profiler ar-rays.
Significant functional improvement and neuroprotection were observed in animals treated immediately or one week after injury, with increased numbers of retrogradely labelled su-pra- and propriospinal neurons compared with controls. Early and one-week-delayed stem cell infusion elevated TIMP-1 levels in the injured spinal cord and spleen, and immediate treatment altered splenic cytokine expression, highlighting the spleen as a key target of stem cell effects.
Our findings indicate that immediate or one-week-delayed intravenous stem cell therapy induces morphological and functional recovery through TIMP-1 modulation.
Funding: This work was supported by Albert Szent-Györgyi Grant provided by the Albert Szent-Györgyi Medical School, University of Szeged to KP (SZGYA-KKA).
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