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MAKING SPECTRAL FIBER PHOTOMETRY EASY: AN OPEN-SOURCE APPROACH TO CAPTURING FLUORESCENT SPECTRA FROM THE LIVING BRAIN
Andrey Formozovand 5 co-authors
Medical Faculty Mannheim, Heidelberg University
FENS Forum 2026 (2026)
Barcelona, Spain
Board PS07-10AM-062
Presentation
Date TBA
Board: PS07-10AM-062
Event Information
Poster Board
PS07-10AM-062
Poster
View posterAbstract
Fiber photometry is a technique that enables real-time monitoring of neuronal activity in molecularly defined populations of neurons in freely moving animals. Its relative simplicity and its flexible combination with optogenetic control of neuronal activity have driven a rapid growth in the popularity of fiber photometry in recent years, leading to novel insights into the fundamental principles of physiological and pathophysiological brain function. At present, most fiber photometry systems detect bulk fluorescence filtered in discrete spectral bands - similar to classical fluorescence microscopy. Capturing the full emission spectrum of fluorescent indicators can provide additional information and thereby expand the capabilities of neuroscientific research. However, the use of conventional spectrometers for fiber photometry in laboratory conditions has proved to be less practical, as they are typically not fully optimized for fiber photometry applications that require large-diameter, high-numerical-aperture fibers. Here, we present a modular, customized hardware solution for spectrally resolved fiber photometry that can be integrated into dichroic-mirror- and fused-fiber-based systems. After thoroughly characterizing our system, we demonstrate spectrally resolved photometry recordings in vivo and explore novel applications that extend the capabilities of conventional fiber photometry.
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