Poster preview
ePoster
METABOLIC TRAJECTORIES IN DEVELOPING HUMAN NEOCORTICAL NEURONS
Gianmarco Ferriand 15 co-authors
Fondazione Pisana per la Scienza
FENS Forum 2026 (2026)
Barcelona, Spain
Board PS03-08AM-446
Presentation
Date TBA
Board: PS03-08AM-446
Event Information
Poster Board
PS03-08AM-446
Poster
View posterAbstract
The shift from glycolysis to oxidative phosphorylation is a key step in neural differentiation. Yet, the timing of metabolic rewiring in the development from progenitors to neurons remains elusive, especially in human corticogenesis. To bridge this gap, here we adopted a unique cell platform based on neocortex-derived human neuroepithelial stem cells, modeling their physiological transition into functional neurons in a four-month longitudinal study and until the acquisition of electrophysiological competence. Using quantitative proteomics, combined with NAD(P)H fluorescence lifetime imaging and metabolomics, we investigated the entire differentiation process and describe how metabolic pathways drive cortical neuron maturation. The prevalent metabolic adaptations were then confirmed in a human neocortical specimen. This study provides a spatio-temporal map of neocortical metabolism during development and offers a framework to investigate human neurometabolic disorders.
Recommended posters
DEVELOPMENT OF CELLULAR ENERGY METABOLISM DURING IN VITRO DIFFERENTIATION OF HUMAN INDUCED PLURIPOTENT STEM CELLS INTO CORTICAL NEURONS
Sarka Danacikova, Petr Pecina, Alena Pecinova, Jan Svoboda, David Vondrasek, Davide Alessandro Basello, Tomas Cajka, Daniel Hadraba, Tomas Mracek, Vladimir Korinek, Jakub Otahal
METABOLIC PROGRAMS AS DETERMINANTS OF NEURAL PROGENITOR STATE AND HETEROGENEITY
Hsin-Jui Lu, Veronika Saharuka, Giulia Di Muzio, Franciscus van der Hoeven, Brittney Armstrong, Gernot Poschet, Erwin Kupczyk, Jakob Meier-Credo, Christiane Opitz, Pei-Chi Wei
MITOCHONDRIA AND METABOLISM IN THE EVOLUTION OF CORTICAL DEVELOPMENT
Enrico Negri, Virginia Fernández, Adrián Cárdenas, Víctor Borrell
METABOLIC BUFFERING BY ASTROCYTES SHAPES CORTICAL MATURATION UNDER MITOCHONDRIAL REDOX STRESS
Eun-Ji Park, Gerta Hoxhaj
A MULTIMODAL APPROACH TO UNDERSTANDING BRAIN ORGANOIDS MATURATION OVER TIME
Filippo Chiesa, Alice Chiodi, Victor Alcolea-Rodriguez, Marcella Bonanomi, Cinzia Cocola, Paride Pelucchi, Chiara Agrimi, Ettore Mosca, Simona Di Lascio, Daniela Gaglio, Diego Fornasari, Renzo Vanna, Eleonora Piscitelli
CHARACTERISING MITOCHONDRIAL REMODELLING DURING NEUROGENESIS
Jordyn Soldin, Erin Buchanan, Caitlyn Mahony, Colleen O'Ryan