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THREE-DIMENSIONAL IMMUNO-HYBRIDIZATION CHAIN REACTION IN THICK, CLEARED TISSUES
Abigail Waltonand 2 co-authors
Karolinska Institute
FENS Forum 2026 (2026)
Barcelona, Spain
Board PS06-09PM-390
Presentation
Date TBA
Board: PS06-09PM-390
Event Information
Poster Board
PS06-09PM-390
Poster
View posterAbstract
Three-dimensional immunohistochemistry (3D-IHC) enables visualisation of protein expression across large biological tissues. Current 3D-IHC approaches typically rely on standard secondary antibody-based detection, which limits multiplexing and often requires long staining times. Immuno-hybridization chain reaction (immunoHCR) couples antibody detection to DNA-based signal amplification, offering a potentially more flexible approach for multiplexed protein visualization. Here, we describe the development of TRIFCO (Tris buffer–mediated retention of immunofluorescence HCR signal in cleared organs), a method designed to integrate HCR signal amplification with 3D antibody staining and tissue clearing. This approach could accelerate staining, enhance signal, and broaden the types of protein-level features and interactions that can be explored in large tissues. By adapting immunoHCR for thick, cleared tissues, TRIFCO provides a potential framework for more comprehensive exploration of protein expression in 3D.
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