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3D TISSUE CLEARING IN NEUROSCIENCE : TO SEE IT BETTER, MAKE IT TRANSPARENT
Samira Osteropand 7 co-authors
Wyss Center for Bio and Neuroengineering
FENS Forum 2026 (2026)
Barcelona, Spain
Board PS01-07AM-415
Presentation
Date TBA
Board: PS01-07AM-415
Event Information
Poster Board
PS01-07AM-415
Poster
View posterAbstract
Three-dimensional histology enables comprehensive visualization and quantitative analysis of intact neural tissues. Tissue clearing has become an essential tool in neuroscience, yet the growing number of available protocols makes method selection increasingly challenging. Here, we first provide an overview of the tissue clearing techniques routinely implemented in our microscopy facility, highlighting their respective strengths and limitations. We then focus on a specific application: quantifying viral expression in the mouse spinal cord. Several clearing strategies were compared, and one was optimized to enhance tissue transparency, antibody penetration, and preservation of endogenous viral fluorescence. This protocol enables whole-spinal-cord imaging using light-sheet microscopy. The resulting volumetric datasets support downstream cell detection and quantitative analysis, including calculation of the proportion of virally transduced motoneurons within the total labelled population. Together, this optimized 3D histological pipeline illustrates how tissue clearing can serve not only as a tool for qualitative visualization but also as a robust framework for quantitative analysis.
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