ePoster

<EM >IN VIVO</EM> DEEP-BRAIN MICROSCOPY AT SUBMICROMETER RESOLUTION WITH REFRACTIVE INDEX-MATCHED PRISM INTERFACES

Chihiro Itoand 13 co-authors

National Institutes for Quantum Science and Technology

FENS Forum 2026 (2026)

Barcelona, Spain

Board PS07-10AM-009

Presentation

Date TBA

View poster

Board: PS07-10AM-009

Poster preview

Event Information

Poster Board

PS07-10AM-009

Poster

View poster

Abstract

The mammalian brain is a thick and densely layered structure comprising a huge number of neurons that work together to process information and regulate brain functions. Although various optical methods have been developed to investigate deep brain dynamics, they are limited by technical constraints, invasiveness, suboptimal spatial resolution, and/or a restricted field of view. To overcome these limitations, we developed an implantable, optically optimized microprism interface with a refractive index matched to that of brain tissue and water, enabling minimally-invasive, wide-field two-photon imaging method with enhanced brightness and sub-micron resolution in deep prefrontal areas.

$We developed a microprism interface, named PRIMISM, with a refractive index matched to that of brain tissue, enabling in vivo deep-brain wide-field imaging at submicrometer resolution. PRIMISM enables imaging of fine dendritic structure and neural population activity at depths exceeding 1 mm in the medial prefrontal cortex of awake mice.$

Recommended posters

A NOVEL ENDOSCOPE DESIGNED FOR RECORDING NEURONAL ACTIVITY IN DEEP BRAIN REGIONS OF FREELY-BEHAVING ANIMALS WITH REDUCED INVASIVENESS

Caio Vaz Rimoli, Claudio Moretti, Stefano Zucca, Fernando Soldevila, Enora Brémont, Serena Bovetti, Sylvain Gigan, Cathie Ventalon

A LONG-WORKING-DISTANCE MINIATURE TWO-PHOTON MICROSCOPE ENABLES CROSS-SPECIES IMAGING FROM RODENTS TO NON-HUMAN PRIMATES

Runlong Wu, Zhu Yufei, Wu Jiali, Hu Yanhui, Wang Aimin, Poo Mu-ming, Zeng Huanhuan, Cheng Heping

MINIMALLY INVASIVE, WIDE-FIELD TWO-PHOTON IMAGING OF THE BRAINSTEM AND NUCLEUS TRACTUS SOLITARII AT CELLULAR RESOLUTION

Azumi Hatakeyama, Daisuke Yamada, Hiroshi Kuniishi, Chihiro Ito, Eri Takeuchi, Shinji Tsuji, Motosuke Tsutsumi, Takako Ichiki, Kohei Otomo, Toshinori Yoshioka, Tomoko Kobayashi, Takamasa Sakai, Atsushi Noritake, Yoshitsugu Aoki, Tomomi Nemoto, Hiroshi Yukawa, Akiyoshi Saitoh, Junichi Nabekura, Masayuki Sekiguchi, Masakazu Agetsuma

A HEAD-MOUNTED MINIATURIZED LIGHT-FIELD MICROSCOPE (MINILFMV2) FOR LARGE-SCALE VOLUMETRIC IMAGING OF NEURAL DYNAMICS DURING NATURAL BEHAVIOR

Tobias Nöbauer, Hao Li, Hossein Sarafraz, Alipasha Vaziri

HIGH-YIELD TWO-PHOTON CALCIUM IMAGING IN FREELY MOVING MICE

Antonio Lorca Camara, Mario Fernandez de la Puebla, Weijian Zong

LONGITUDINAL THREE-PHOTON IMAGING OF NEURAL STEM CELL DYNAMICS IN THE INTACT MOUSE BRAIN

Yusaku Hontani, Xin Su, Yicheng Wu, Diana Machado, Megumi Mizoguchi, John Darby Cole, Hansjörg Kasper, Philipp Bethge, Fritjof Helmchen, Sebastian Jessberger