Our aim is to reveal the relationship between synaptic structural plasticity and turnover and the remodelling of perisynaptic astrocytic processes in vivo, and to identify the relevant signalling cascades and the consequences of their disrup-tion. The project relies on an extensive experimental toolset for monitoring synaptic and astrocytic structure and its dynamic changes using multiphoton fluorescence microscopy and for manipulating astrocyte morphology. In addition, a broad spectrum of imaging approaches and electrophysiological methods is available to dissect this further.