Topic: AAV8

The salamander axolotl (Ambyostoma mexicanum) is a model for studying nervous system evolution, development, and regeneration. Axolotls can regenerate injuries to the central and peripheral nervous system making them an ideal model to study formation and regeneration of neuronal circuits. Tools to visualize and manipulate cells of the axolotl nervous system with high efficiency, spatial and temporal precision are therefore greatly required. Recombinant adeno-associated viruses (AAVs) are frequently used for in vivo gene transfer of the nervous system but virus-mediated gene delivery to the axo

Many neuroscience studies in rodents are limited spatially and temporally by the use of tethered devices, head-fixed recordings or battery-powered telemetric implants. When subtle behavioural readouts are needed, protruding implants may affect neurophysiological and psychobehavioural parameters. We have successfully deployed and continue to develop ADInstruments’ KAHA Wireless Optogenetics Biopotential Telemeter which allows simultaneous user-defined optogenetic stimulation (460nm) and continuous electroencephalogram recording at 2kHz. Powered by inductive charging to remove the limitations of