Topic: acute rat hippocampal slices

Transcranial focused ultrasound (tFUS) targets deep brain areas by non-invasively administering low intensity ultrasound through the brain with a high spatial resolution (Zhang et al., 2021). Previous results indicate that stimulating neural cells at specific pulsing frequencies is neuroprotective (Manippa et al., 2022). We were therefore interested in determining whether a similar approach could be taken with focused ultrasound stimulation. To this end, we prepared acute rat hippocampal slices and exposed them to 40Hz pulsing ultrasound for 1hr. Following a proteomics screen, we noted an incr

Understanding synaptic connectivity is crucial for understanding how neuronal networks contribute to specific brain functions. However, current methods can measure the functionality only of a limited number of synaptic connections in sufficient detail. Therefore, we evaluated the applicability of a voltage imaging method in acute rat hippocampal slices to map unitary synaptic connections between CA3 neurons by using Voltron, which can detect both action potentials and small subthreshold events, such as unitary EPSPs. These connections are crucial for major hippocampal memory functions; however