Topic: alpha-synuclein pathology

We aimed to develop and standardize a diet and rotenone-based animal model of Parkinson's disease. To achieve this, 21 days old male C57BL/6 mice were fed on a high-fat diet [HFD] (60%kcal) for 17 weeks; starting from week 11, animals were orally gavaged daily with 10mg/kg rotenone dose till the end of the 17th week. Further, they were subjected to behavioral tests (Rota-rod, Y maze, and fecal pellet output test). Fecal pellets were collected for 16S rRNA sequencing and animals were subsequently euthanized. Our data revealed that rotenone and its combination with HFD exhibited loss of motor fu

Parkinson’s disease (PD) is a progressive neurodegenerative disorder. Deep brain stimulation (DBS) of the subthalamic nucleus (STN) is a common target for treating PD's motor symptoms. However, we have little understanding of STN pathology in PD. We aimed to examine the cellular and functional pathology of the STN in PD using both rodent and post-mortem human brain tissue. First, we show bidirectional motor control by STN using optogenetic modulation, where inhibition of the STN increases movement and STN activation decreases movement. We also examined the effect of bidirectional optogenetic m