Topic: APV

In the brain, the neuronal activity is kept and controlled by a precise and well-defined “Excitatory/Inhibitory balance” among neurons, which holds importance in neurodegenerative and neurodevelopmental disorders. With the final aim of generating a reliable in vitro system of the human brain, this work was focused on the interplay between excitatory (E) and inhibitory (I) neurons in neural networks derived from human induced pluripotent stem cells (hiPSCs), deepening the critical role of heterogeneity. We exploited high-density Micro-Electrode Arrays (MEAs, 2304 electrodes) to explore two neur

In vitro models combined with human induced pluripotent stem cells (hiPSCs) are a widely used tool to investigate the complex mechanism of the human brain. Indeed, the use of hiPSCs-derived neuronal networks allow to create in vitro patient-specific models that most closely resembles the processes of the human system, providing a reliable model to test neuroactive properties of different compounds. This study investigates how different drugs affected the electrophysiological activity of in vitro systems composed by excitatory (E) and inhibitory (I) neurons derived from hiPSCs. Our experimental