Topic: assembloids

Studying human brain development has remained difficult as appropriate models lack. Recently, induced pluripotent stem cells (iPSC)-derived 3D brain organoids have been developed. They recapitulate the key aspects of human brain development such as the cellular complexity and functional properties of specific regions. Yet, longitudinal functional studies are sparse. During neurodevelopment in vivo, GABAergic inhibitory interneurons from the ventral cortex migrate to populate the dorsal part of the cortex enriched in glutamatergic excitatory neurons (Zhu et al. Neuron, 1999; Le et al., JBC, 200

The intricate development of the visual system involves spatial and temporal coordination, especially the projection of retinal ganglion cell (RGC) axons to post-synaptic targets in the brain. Although retinal organoids (ROs) have mimicked retinal development, their limitations in modeling RGC projections prompted the creation of in vitro assembloids integrating retinal and brain organoids. This study aims to elucidate the projection patterns of the human visual system of inherited retinal dystrophy (IRD) subjects and how this might influence RGC development and survival while providing answer