Topic: astrocytic markers

Air pollution is a significant threat to human health and it is estimated that globally 8.9 million deaths happen because of it. A link between urban particulate matter exposure and an increased risk of neurodegenerative disease has already been identified. For this reason, I used wild-type hiPSCs to set up a 2D differentiation protocol of about 70 days. Cultures were untreated or treated with two doses of manganese, 1 μM and 20 μM, chosen in relation to scientific literature and after a MTS viability assay. Treatments started from day 40 until the end of differentiation (intermediate time poi

The nucleus accumbens (NAc), a brain region implicated in the regulation of anxiety-related behaviors, has been found to be susceptible to blood brain barrier (BBB) permeability in psychopathologies. Additionally, the positive effects of testosterone on BBB function and anxiety related behaviours is still controversial. In this study, exploiting natural phenotypic variation in anxiety-like behaviors in outbred Wistar male rats, we aimed at investigating whether testosterone plays a role in anxiety-related BBB disfunction. We classified animals as high (HA) or low anxious (LA) according to the