Topic: awake head-fixed mice

Ultra-thin holographic endo-microscopes exploiting multi-mode optical fibres (MMF) have recently emerged as powerful tools, allowing us to explore the deepest regions inside living mouse brains with negligible disruption and diffraction-limited resolution [1,2,3]. Recently published work representing current state-of-the-art holographic endo-microscopy deals with detailed imaging of neuronal structures, functional monitoring of red blood cell velocity and calcium dynamics located in the deepest regions of anaesthetized mice [4]. In addition to technological development, the transition to imagi

Visual coding is a highly dynamic process and continuously adapts to the current viewing context. At early stages of visual processing, adaptation was generally studied without considering behavioural contexts. However, we recently learned that locomotion and arousal strongly modulate visual processing, even at the retina level. Here, we aim to understand how locomotion impacts visual adaptation in the superior colliculus (SC), the most prominent visual centre in mice, which integrates retinal input in its superficial layers and performs sensorimotor transformations in its deeper layers. We r

The medial prefrontal cortex (mPFC) is important for higher cognitive functions. In vivo recordings of neuronal activity in the mPFC have been achieved via invasive electrical and optical approaches. Recently developed three-photon (3P) in vivo imaging enables deeper tissue penetration than two-photon microscopy. Here, we demonstrate high resolution structural and functional 3P-imaging in the mPFC of the mouse at unprecedented depth. Specifically, we perform Ca2+-imaging in awake head-fixed mice up to a depth of 1700 µm in the mPFC. We record Ca2+-transients in neurons, astrocytes and longitud

The state-of-the-art techniques for all-optical interrogation of neuronal circuits in vivo typically combine two-photon (2P) calcium imaging and digital holography to precisely activate target neurons with sculpted light patterns. This approach is however limited in speed and in throughput, ultimately due to the intrinsic constraints of digital holography. We employed acousto-optic deflectors (AOD)-based 2P approaches (“ULoVE”, Villette et al. 2019; “3D-CASH”, Akemann et al. 2022) to simultaneously record and perturb neuronal activity in awake head-fixed mice across single planes or three-dime