Topic: axonal projections

The mammalian Prefrontal Cortex (PFC) has been suggested to modulate sensory information processing across multiple cortical regions via long-range axonal projections. These axonal projections arise from discrete PFC subregions with unique brain-wide connectivity and functional repertoires, which may provide the architecture for modular feedback intended to shape sensory processing. Here, we used axonal tracing, axonal and somatic 2-photon calcium imaging, and chemogenetic manipulations in mice to delineate how projections from the Anterior Cingulate Cortex (ACA) and ventrolateral Orbitofronta

The basolateral amygdala (BLA) is a cortical structure playing a role in various cognitive processes. In spite of many studies focusing on local information processing within the circuits of the BLA, the features of amygdalar principal neurons (PNs) remained elusive. Here, we combined neuroanatomical, electrophysiological and tracing techniques to determine the single-cell features, dendritic and axonal projections of PNs within the lateral (LA) and basal amygdala (BA). Using a mouse reporter line, we found that cholecystokinin (CCK) expression defined two groups of spatially segregated PNs bo

The brain continuously receives a wealth of sensory information, and uses this information to guide behaviour. However, the process by which different sensory streams are integrated to form a unified percept remains poorly understood. Cortical regions dedicated to a specific sensory modality nonetheless receive input related to other modalities. The mouse auditory cortex sends numerous projections to the primary visual cortex and surrounding higher visual areas. These inputs may be crucial to the integration and binding of auditory and visual signals. Yet the exact nature of the auditory infor

Huntington’s disease (HD) is a currently incurable neurodegenerative disorder that manifests itself through motor and cognitive symptoms due to a predominant loss of striatal projection neurons (SPNs) in the striatum. Although HD is a neurodegenerative disease, growing evidence show that developmental alterations could be a key factor in the disease, determining the future vulnerability of certain cell types, such as SPNs. Here we show an early HD-related gene expression pattern elucidated by bulk and single-cell RNA sequencing performed on wt and HD isolated mouse striatal primordia at differ

The corticospinal tract (CST) facilitates skilled, precise movements, which necessitates that corticospinal neurons (CSN) establish segmentally specific connectivity with spinal circuitry. Recent work has identified CSN-intrinsic molecular determinants that regulate CSN axonal projections to specific segmental levels of the neuraxis and that these are expressed by CSN subpopulations that project to distinct levels of the neuraxis. In the present study, we establish the use of a newly generated Klhl14-T2A-Cre knock-in reporter mouse line to investigate axonal projections of a specific CSN subpo