Topic: bicuculline

While dissociated cultures of rodent neurons on microelectrode arrays (MEAs) have become a prominent method for studying neuronal network properties, establishing human neuronal cultures with mature electrophysiological features compatible with MEAs has posed a significant challenge. The aim of this study was to establish a reliable protocol for generating human-derived neuronal networks on High Density Microelectrode Arrays (HD-MEA) and to characterize their electrophysiological features for potential applications in disease modeling and therapeutic testing. Human-induced pluripotent stem cel

Tourette syndrome (TS) is a neurodevelopmental disorder characterized by motor and vocal tics, which are sudden, rapid, recurrent, non-rhythmic movements. The TS is also accompanied by cognitive and emotional dysfunctions such as obsessive-compulsive disorder (OCD) and attention deficit hyperactivity disorder (ADHD). Dysfunction of the cortico-basal ganglia-thalamocortical circuits has been suggested to induce these symptoms in TS patients, but the details of neuronal mechanisms underlying tic disorders remain unknown. In this study, we generated drug-induced tic model mice by unilateral local

In this study, our objective was to explore the role of GABAA receptors within the amygdala region in modulating anxiety-like behavior in response to voluntary physical activity. We employed a stereotaxic instrument to implant two cannulae in the amygdala area, through which we administered the GABAA receptor antagonist, bicuculline. Subsequently, we exposed the animals to a running wheel for a duration of three hours over four weeks. Following this exercise regimen, we conducted behavioral tests, including the open field and elevated plus maze tests, to assess anxiety-like behavior. The findi

Muscimol is a potent agonist of inhibitory GABAA-receptors, which are a heterogenous family of pentameric complexes. Muscimol is considered to bind to all receptor subtypes at the orthosteric β+/α- interface. In one of our recent publications, we observed that the antipsychotic drugs clozapine (CLZ), loxapine (LOX) and chlorpromazine (CPZ) showed diverging results in displacing 3H-muscimol. While a complete displacement could be observed by bicuculline (BIC), and no displacement with CPZ, the compounds CLZ and LOX could only incompletely compete with the radioligand. In addition, the dose-resp

GABAergic neurotransmission is enhanced in the cerebellum of hyperammonemic rats, an animal model of hepatic encephalopathy, and this induces motor incoordination. There is an interplay between neuroinflammation and GABAergic neurotransmission, but the underlying mechanisms remain unclear. This work aimed to unveil the molecular mechanisms by which GABAergic neurotransmission modulates neuroinflammation in the cerebellum.Ex-vivo experiments were performed using cerebellar slices from control and hyperammonemic rats treated or not with bicuculline. We analyzed by immunohistochemistry microglia

In vitro models combined with human induced pluripotent stem cells (hiPSCs) are a widely used tool to investigate the complex mechanism of the human brain. Indeed, the use of hiPSCs-derived neuronal networks allow to create in vitro patient-specific models that most closely resembles the processes of the human system, providing a reliable model to test neuroactive properties of different compounds. This study investigates how different drugs affected the electrophysiological activity of in vitro systems composed by excitatory (E) and inhibitory (I) neurons derived from hiPSCs. Our experimental