Topic: c-Fos+ cells

Memory mechanisms remain elusive in their system implementation. Previous research, combining fMRI and electrophysiology in anaesthetized rats, has shown that synaptic plasticity in the Dentate Gyrus (DG) orchestrates coordination in memory-related brain networks. By employing graph theory and pharmacogenetic tools, we further unveiled a hierarchy in system-level communication that highlighted interactions between the hippocampus, nucleus accumbens, and prefrontal cortex. However, whether a similar functional architecture supports brain coordination in awake behaving animals remains unknown.Us

Automatically detecting antibody labelled cells within three-dimensional datasets such as whole-brain image stacks generated with light-sheet fluorescence microscopy is a complex task. In this study, we introduce DELiVR, a virtual reality (VR) trained deep learning pipeline for identifying c-Fos+ cells, which serve as markers of neuronal activity, in cleared mouse brains. By employing VR annotation, we significantly accelerated training data generation, resulting in DELiVR surpassing current state-of-the-art methods for cell segmentation. Our pipeline, which encompasses cell detection, brain a