CA1 pyramidal neurons

Dendrites perform non-linear processing of synaptic inputs, and drive active processes such as plateau potentials which are critical to computation and plasticity. Therefore, the ability to image voltage across an entire dendritic tree would represent a major advance in systems neuroscience. Recent advances in genetically encoded voltage indicators (GEVIs) have brought this possibility closer to reality. However, due to the fundamental tradeoff between imaging speed and volume, it has been impossible thus far to image an entire dendritic tree using volumetric imaging at high speeds. To get aro

…Specifically, we focus on CA1 pyramidal neurons, which receive inputs from the CA3 and entorhinal cortex (EC). No widely accepted network-based theory for place field formation exists, but many models offer mechanisms for place-field firing. One type of hypotheses proposes that it results from summated output of spatially tuned cells, like grid cells or boundary vector cells. Another suggests CA1 place fields are replicate of CA3 place fie

Aim: Acid-sensing ion channels 1a (ASIC1a) are widely distributed in the mammalian nervous system and in particular brain areas including the hippocampus. ASIC1a is highly permeable to Ca2+ and its activation has a crucial importance in numerous physiological and pathological processes, including synaptic plasticity, learning and memory. To further understand the role of ASIC1a channels in the synaptic alterations induced by Aβ, we carried out electrophysiological experiments investigating the effects of a ASIC1a-blocking monoclonal antibody ASC06-IgG on hippocampal long-term potentiation (LTP

Among the genomic alterations identified as risk factors in Autism Spectrum Disorders (ASD), haploinsufficiency of AMBRA1 (Activating Molecule in Beclin1-Regulated Autophagy) triggers an ASD phenotype associated with hippocampal hyperexcitability exclusively in the female sex. Remarkably, AMBRA1 protein is constitutively more expressed in the hippocampus of wild-type (Wt) females than males. However, being more decreased by AMBRA1 deficiency in the female than the male sex, it results comparably expressed in the hippocampus of autistic females and non-autistic males. In this study, we verified

The postsynaptic site of excitatory synapses is characterized by an electron-dense region called postsynaptic density (PSD), which is usually located at the tip of dendritic spines. The PSD has been shown to undergo morphological changes in response to behavioral and electrophysiological inputs. This process can be highly dynamic and occurs over different time scales. To bridge the observation gap between short-term plastic events (ranging from minutes to hours) and long-term effects (several hours to days), we engineered a novel on stage microscope-incubator optimized for long-term culturing

The electrical behavior of neurons depends on their morphology as well as various biophysical characteristics including passive membrane and intracellular properties and the identities and densities of voltage-gated ion channels. However, some of these parameters are often not sufficiently constrained by the available experimental data and need to be inferred from the response of the neuron to specific external stimuli. Data-driven models have proved to be useful in this context; one approach is to tune the unknown parameters in the model until the output of the model best approximates the tar

…which a large number of hippocampal pyramidal neurons fire synchronously. Recent studies indicate that the participation of hippocampal pyramidal neurons in SWRs is variable. The variability may be explained by gene expression pattterns. However, there are no adequate methods to comprehensively analyze these molecular properties of a single neuron in the context of its electrophysiological spike patterns. Here, we developed an experimental technique t

…of sodium and calcium, which damages neurons. Therefore, deletion of NMDA receptor subunits may reduce cell death induced by acute brain injury. MK801 is a non-competitive NMDA receptor antagonist that can block NMDA ion channels. In this experimental study, Wistar rats were divided into 6 groups:Sham, I/R, I/R+NMDA (0.5 mg/kg, IV), I/R+MK801 (0.5 mg/kg, IV), I/R+NMDA+ electrophysiology, and I/R+MK801+ electrophysiology. The acute cerebral ischemic injury was induced in rats by o

…explore this hypothesis, we birthdated CA1 pyramidal neurons using in-utero electroporation and examined their functional featu

…We previously showed that CA1 pyramidal neurons of the same embryonic birthdate exhibit prominent cofiring across different brain states and overlapping place tuning during behavior (Author et al.,

In behaving rodents, CA1 pyramidal neurons receive spatially-tuned depolarizing synaptic input while traversing a specific location within an environment called its place. Midbrain dopamine neurons participate in reinforcement learning, and bursts of action potentials riding a depolarizing wave of synaptic input signal rewards and reward expectation. Interestingly, slice electrophysiology in vitro shows that both types of cells exhibit a pronounced reduction in firing rate (adaptation) and even cessation of firing during sustained depolarization. We included a five state Markov model of NaV1.6

The CA1 pyramidal neurons are embedded in an intricate local circuitry that contains a variety of interneurons. The roles these interneurons play in the regulation of the excitatory synaptic plasticity remains largely understudied. Recent experiments showed that repeated cholinergic activation of 𝛼7 nACh receptors expressed in oriens-lacunosum-moleculare (OLM𝛼2) interneurons could induce LTP in SC-CA1 synapses. We used a biophysically realistic computational model to examine mechanistically how cholinergic activation of OLMa2 interneurons increases SC to CA1 transmission. Our results suggest t