Topic: Ca2+ transients

The medial prefrontal cortex (mPFC) is involved in cognitive and emotional functions housing neurons and astrocytes, impacting mPFC activity. To this end, astrocytes express several GPCR to fine-tune synaptic transmission such as CB1R and adenosine A1 and A2A receptors (A1R, A2AR). Since interactions of these receptors in astrocytes are not known, this work aims to fill the gap of the crosstalk between CB1R and adenosine receptors and their contribution for mPFC synaptic plasticity.CB1R-A1R and CB1R-A2AR complexes were observed in primary cultures of astrocytes, by using Proximation Ligation a

[Aims] Neurons in mouse visual cortex encode high-dimensional sensory information and lower-dimensional behavioral variables. Here we asked how behavioral signals are organized across the cortical volume, and whether they follow the organization of visual activity.[Methods] We used Light Beads Microscopy (Demas et al, 2021) to functionally image Ca2+ transients at cellular resolution from large volumes of cortex (3 x 3 x 0.5 mm^3) in awake, headfixed transgenic mice expressing GCaMP6s. To segment individual cells, we developed a novel volumetric cell extraction pipeline by accelerating and ext

Astrocytes play a fundamental role in numerous neural processes. In this study, we present preliminary data from ex vivo human cortical astrocytes obtained from Temporal lobe resections for epilepsy treatment and examined using whole cell patch clamp and 2-photon imaging techniques. Calcium (Ca2+) imaging reveals that these astrocytes exhibit a high frequency of Ca2+ transients in their fine processes compared to whole-cell responses, a finding consistent with astrocyte activity recorded in rodents. While the morphology of human astrocytes bore similarities to their rodent counterparts, they d