Topic: CA3 neurons

Research into the hippocampus has long emphasized its plasticity (McClelland et al., 1995), though recent reports have highlighted its remarkably stable firing patterns (Mizuseki et al., 2013). How plasticity updates networks maintaining their dynamics remains an open question, largely due to a lack of experimental access points into network stability. Development may provide one such access point. We previously showed that CA1 pyramidal neurons of the same embryonic birthdate exhibit prominent cofiring across different brain states and overlapping place tuning during behavior (Author et al.,

Although changes in synaptic efficacy are widely thought to be a substrate for memory storage in the brain, the rules of synaptic plasticity are difficult to assess in vivo and thus remain unclear. We considered the dynamics of hippocampal representations as an indirect indicator of ongoing plasticity during memory formation and familiarization. Using calcium imaging data from mice navigating virtual tracks, during which CA1 or CA3 neurons were recorded, we tracked the evolution of the spatial modulation of their activity (place fields) lap by lap. During exploration of a novel environment, 50

The axon initial segment (AIS) is an axonal subdomain integral to neuronal excitability, yet its geometrical diversity within the hippocampus is poorly understood. This study provides a detailed examination of the variability of proximal cell morphology across murine hippocampal neurons, analyzing molecular and geometrical parameters such as AIS length and position, dendrite shape, soma, and apical dendrite size across dorsal-ventral, superficial-deep, and proximal-distal axes. We observed significant differences in AIS characteristics, with CA3 neurons displaying longer AIS and more distal or

Understanding synaptic connectivity is crucial for understanding how neuronal networks contribute to specific brain functions. However, current methods can measure the functionality only of a limited number of synaptic connections in sufficient detail. Therefore, we evaluated the applicability of a voltage imaging method in acute rat hippocampal slices to map unitary synaptic connections between CA3 neurons by using Voltron, which can detect both action potentials and small subthreshold events, such as unitary EPSPs. These connections are crucial for major hippocampal memory functions; however