Topic: carbachol

The hippocampus is essential for spatial navigation and memory processes. Hippocampal pyramidal cells (PCs) often exhibit complex spike bursts (CSBs) that are driven by regenerative dendritic Ca2+ plateau potentials. These events can induce rapid synaptic plasticity and new place field (PF) formation in CA1PCs. CA3PCs also fire CSBs, and their PF-inducing CSBs were shown to be particularly prolonged (Li et al, bioRxiv). However, the dendritic mechanisms underlying such long-lasting events in CA3PCs remain unclear. In acute rat brain slices, we observed large heterogeneity among CA3PCs in CSB p

Sleep is a recurring state of inactivity in which the brain undergoes a period of rest and restoration; its disturbances can severely affect health. Sleep and wake cycles can be mimicked in vitro: cortical neurons plated on Micro-Electrode Arrays (MEAs) show spontaneously synchronized, low-frequency firing patterns resembling the slow wave oscillations typical of NREM sleep. With specific compounds (e.g., Carbachol, orexin), network activity can be manipulated to switch to a wake state. To reduce the costs and ethical issues related to in vitro studies and to be more sustainable in research, i

The different behavioral states inherent to the wake-sleep cycle are reflected in the electrical activity of the cerebral cortex. Slow-wave sleep (SWS) and deep anesthesia are dominated by high amplitude slow-wave oscillations (SWO, < 1 Hz). With the increase in arousal, desynchronization and low-amplitude fast rhythms arise. While SWS and wakefulness dynamics have been comprehensively described, their transition remains relatively unexplored. Tort-Colet et al (2021) previously reported the emergence of an intermediate brain state, the microarousal brain state, characterized by the rhythmic