Topic: Cav2.1[EFb]

ePoster: 2 ePosters

Alternative splicing of Cav2.1 EF-hand contributes to the tightness of calcium influx-neurotransmitter release coupling at mouse cerebellar synapses

Kohgaku Eguchi, Le Monnier Elodie, Ryuichi Shigemoto · FENS Forum 2024

The coupling of voltage-gated calcium channels (VGCCs) and vesicular calcium sensors for neurotransmitter release is crucial for fast and efficient synaptic transmission. At parallel fiber (PF)-Purkinje cell (PC) synapses in mouse cerebellum, the coupling tightens during synaptic maturation in parallel with switching of splicing isoforms of the P/Q-type VGCC (Cav2.1) EF-hand from EFb to EFa. The Cav2.1[EFa] isoform has been reported to have a tighter Ca2+ influx-sensor coupling distance than the Cav2.1[EFb] isoform in hippocampal pyramidal neurons. Thus, the shift in the splicing Cav2.1-EF han

ePoster · Neuroscience

Cav2.1 splice variants organize in nanodomains at presynaptic boutons where they differentially regulate synaptic vesicle release and synaptic facilitation

Asja Ragnini, Agnes Thalhammer, Fanny Jaudon, Jessica Muià, Lorenzo A. Cingolani, Gabriele Baj · FENS Forum 2024

Cav2.1 channels are critically important for fast synaptic transmission at central synapses. The two Cav2.1 isoforms Cav2.1[EFa] and Cav2.1[EFb] are generated by alternative splicing of two mutually exclusive exons, 37a and 37b. Both isoforms are expressed throughout the adult brain, whereas Cav2.1[EFb] predominates at early stages of development. Cav2.1[EFa] supports synchronous release and short-term synaptic depression, whereas Cav2.1[EFb] boosts asynchronous release and short-term facilitation. We investigated whether the two isoforms were distinctly located at excitatory nerve terminals r

ePoster · Neuroscience