Topic: CB1R

The medial prefrontal cortex (mPFC) is involved in cognitive and emotional functions housing neurons and astrocytes, impacting mPFC activity. To this end, astrocytes express several GPCR to fine-tune synaptic transmission such as CB1R and adenosine A1 and A2A receptors (A1R, A2AR). Since interactions of these receptors in astrocytes are not known, this work aims to fill the gap of the crosstalk between CB1R and adenosine receptors and their contribution for mPFC synaptic plasticity.CB1R-A1R and CB1R-A2AR complexes were observed in primary cultures of astrocytes, by using Proximation Ligation a

AIMS: The activation of CB1 receptor (CB1R) is reported to favor energy accumulation and promote resiliency against stress. The aim of this study is to elucidate whether CB1R expression in CaMKII+ neurons is sufficient to restore its role in the regulation of energy metabolism and contingent behaviors.METHODS: To this end, we applied a genetic strategy to reconstitute CB1R expression at endogenous levels in CaMKII+ neurons (CaMKII-CB1-RS).RESULTS: The rescue of CB1R expression in these neurons promotes feeding behavior causing fasting-induced hyperphagia, increased motivation and impulsivity t

Intricate and extended mechanisms are in place to allow for repair following injuries of the peripheral nervous system (PNS), particularly at the neuromuscular junction (NMJ). Type 1 endocannabinoid receptors (CB1R) may be involved in the repair process owing to their presence in the PNS and at the NMJ and given their role in axonal growth and neuronal plasticity. Owing to the role of perisynaptic Schwann cells, glial cells at the NMJ, in the regulation of NMJ repair following injury, we postulate that CB1R participate in NMJ denervation and reinnervation processes and that this regulation may

Incidental learning (IL) can be studied using sensory preconditioning, a higher-order memory process. Incidental associations between low-salience stimuli (e.g. odors, tastes, lights or tones) happen during a preconditioning phase that is followed by classical conditioning of one of these stimuli with an aversive or appetitive unconditioned reinforcer. As a result, subjects present a response to the directly conditioned stimulus but also a mediated response to the preconditioned stimulus never explicitly reinforced, implying initial IL. We performed three different protocols to study IL using

Consumption of cannabinoid-containing products has increased for their beneficial effects on pregnancy for reducing symptoms such as nausea, vomiting or anxiety. However, FDA clearly strongly advises against the use of delta-9-tetrahydrocannabinol (THC) during pregnancy. Alongside, mostly of the studies have investigated the effects of prenatal THC (pTHC) exposure, in males only, but pTHC shows a sex bias. Here, we investigate the effects of pTHC exposure in the male and female young rats.Sprague and Dawley rats were subcutaneously treated with THC (2 mg/kg/day) or vehicle (VHC), from gestatio

There is intense scientific, medical, and societal interest in the endocannabinoid system (ECS) because it modulates a wide range of brain functions including mood, appetite/energy expenditure, and learning and memory. However, because of the complex and pleotropic pharmacology of the ECS, cannabinoid drugs are associated with unwanted neurological and psychoactive side effects. These limitations drive the need for increased biochemical and cell biological understanding of the ECS and a reappraisal of how this system may be targeted therapeutically.Endocannabinoid signalling mediated by cannab

Early life stress (ELS) can induce long-term changes of brain functions and behavior, thus representing a significant risk factor for the development of mental disorders such as depression and anxiety. A brain system particularly affected by ELS is the endocannabinoid system, a critical regulator of socio-emotional behavior and the stress response. In a first study we showed that adult female rats exposed to ELS show significant changes in their behavioural phenotype, specifically depressive-like symptoms such as anhedonia and disturbed social behavior. These behavioral changes where parallele