Topic: cellular architecture

Traditional histology relies on thin sections of small samples for studying human tissues, which limits our understanding of the complex structure and anatomical relationships of whole organs. Gross anatomy and medical imaging on the other hand lack the resolution and specificity to visualize histological structures. Tissue clearing methods are emerging as innovative solutions to transcend this constraint by revealing the spatial architecture of large tissues samples. However, making adult human organs transparent remains a challenge, given their size, density and endogenous fluorescence. We h

The amygdala, or an amygdala-like structure, is found in the brains of all vertebrates and plays a critical role in survivaland reproduction. However, the cellular architecture of the amygdala and how it has evolved remain elusive. Here, we generated single-nucleus RNA-sequencing data for more than 200,000 cells in the amygdala of humans, macaques,mice, and chickens. Abundant neuronal cell types from different amygdala subnuclei were identified in all datasets. Cross-species analysis revealed that inhibitory neurons and inhibitory neuron-enriched subnuclei of the amygdala were well-conserved i