Topic: cerebellar astrocytes

The mammalian brain stores glucose, the main energy substrate from the blood circulation, in the form of glycogen. In the rodent brain, the cerebellum stores relatively large amounts of glycogen, yet the cellular and subcellular distribution of glycogen has not been described in detail. Using monoclonal antibodies against glycogen, we investigated the glycogen distribution in the mouse cerebellar cortex. We found a dominant presence of glycogen in molecular layer Bergmann glia (BG) processes. Additionally, glycogen was observed in Purkinje cells (PCs), the principal neurons of the cerebellar c

Astrocyte heterogeneity is essential for understanding the development and function of the central nervous system. While the main types of cerebellar astrocytes can be identified based on their morphology and location, we have limited knowledge about their molecular profile, development, and functions. Furthermore, whether the existing classification truly reflects the full extent of cerebellar astrocyte heterogeneity remains unclear. Single-cell RNA sequencing and spatial transcriptomics performed on both postnatal and adult mouse cerebella unveiled the transcriptome of established astrocyte